Investigating a Functionalized Terthiophene Surface for the Detection of Progesterone Using Surface Plasmon Resonance
نویسندگان
چکیده
Herein we report a rapid and sensitive surface Plasmon resonance (SPR) biosensor to detect progesterone (P4), a model analyte of small molecules. A terthiophene scaffold with an oligoethylene glycol (OEG) linker is used as an anchor for the P4-ovalbumin (OVA) immobilization on gold to form a stable, regenerable SPR surface. Subsequently, inhibition SPR immunoassays based on this terthiophene/OVA-P4 surface have demonstrated that P4 limit of detection (LOD) in buffer solution was 0.13 ng ml. Due to the rapidity of each measurement (less than 5 min per measurement), and the high sensitivity of the assay, we believe the sensing platform may hold great potential for P4 detections.
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